Forensic DNA Typing of Old Skeletal Remains Using AmpFlSTR®Identifiler® PCR Amplification Kit

STR-typing of ancient skeletal remains: which multiplex-PCR kit is the best?

AIM To comparatively test nine co mmercially available short tandem repeat (STR)-multiplex kits (PowerPlex 16, 16HS, ES, ESI17, ESX17, S5 [all Promega]; AmpFiSTR Identifiler, NGM and SEfiler [all Applied Biosystems]) for their efficiency and applicability to analyze ancient and thus highly degraded DNA samples. METHODS Fifteen human skeletal remains from the late medieval age were obtained an...

PCR Typing of Trichophyton Rubrum Isolates by Specific Amplification of Subrepeat Elements in Ribosomal DNA Nontranscribed Spacer

Background: Trichophyton rubrum (T. rubrum) is the most common cause of dermatophytosis of skin and nail tissue. Strain identification in Trichophyton rubrum is important for identification of strain-related differences in infectivity potential or transmissibility and epidemiological studies. PCR typing could determine whether the original isolate is responsible for re-infection or a new strain...

Forensic DNA Typing: Quo Vadis?

Since Alec Jeffreys coined the term “individual-specific genetic fingerprints” in 1985, DNA typing has become indispensable in forensic analysis, having as its central rationale the evidential power of the “match” between trace evidence and suspect. Established technological advances in this field include the adoption of the polymerase chain reaction (PCR) to generate DNA profiles from minute b...

The forensic evaluation of burned skeletal remains: a synthesis.

In recent years, research and case experience have greatly augmented knowledge regarding the effects of extreme heat on skeletal remains. As a result of this effort, enhanced interpretation is now possible on such issues as the extent of recovery, reconstruction, trauma, individual identification, size reduction, thermal effects on histological structures, color variation, the determination if ...

Age Estimation of Fetal Skeletal Remains from the Forensic Context